Medical Mycology Research Center, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba 260-8673, Japan.
Microbiol Immunol. 2010 Jun;54(6):368-70. doi: 10.1111/j.1348-0421.2010.00225.x.
T In our previous studies, TPI were found to be the molecules responsible for contact-killing of C. neoformans by S. aureus cells. Since TPI is a glycolytic protein that functions in the cytoplasm, evidence that TPI is present on the surface of S. aureus was required. In the present study, the presence of TPI on the cell surface of S. aureus was demonstrated by agglutination test and scanning immunoelectron microscopy. Furthermore, TPI was found to be present at a lower density than protein A/G molecules on the surface of S. aureus.
在我们之前的研究中，TPI 被发现是金黄色葡萄球菌细胞接触杀伤新型隐球菌的分子。由于 TPI 是一种在细胞质中发挥作用的糖酵解蛋白，因此需要证明 TPI 存在于金黄色葡萄球菌的表面。在本研究中，通过凝集试验和扫描免疫电子显微镜证明了 TPI 存在于金黄色葡萄球菌的细胞表面。此外，还发现 TPI 存在于金黄色葡萄球菌表面的密度低于蛋白 A/G 分子。
