Raddatz E, Monnet-Tschudi F, Verdan C, Kucera P
Institute of Physiology, Faculty of Medicine, University of Lausanne, Switzerland.
Anat Embryol (Berl). 1991;183(1):57-65. doi: 10.1007/BF00185835.
The distribution of the fibronectin-rich extracellular matrix (ECM) in the chick embryo during formation of the blastula has been evaluated semiquantitatively using an electron microscopical immunogold staining technique. During the first 10 h of postlaying development, fibronectin was found in both embryonic area pellucida and extra-embryonic area opaca of the blastoderm. In the area pellucida, the fibronectin was (1) associated with the basal lamina of the epiblast, (2) present between epiblastic and hypoblastic cells and (3) occasionally internalized in hypoblastic cells. Along the embryonic axis, a transient and high density of ECM was associated with the front of the anteriorly and rapidly expanding hypoblast. Very high density of fibronectin was observed in the marginal zone of the area pellucida, where the epiblastic and deeper cell layers show contacts and intense re-arrangements. In the area opaca, fibronectin was at first found only sporadically between contacting cells, but its density increased steadily and markedly during the first day of development. These rapid and significant changes in the regional distribution of fibronectin-rich ECM are discussed with respect to the early morphogenesis of the chick embryo.
利用电子显微镜免疫金染色技术，对囊胚形成过程中鸡胚富含纤连蛋白的细胞外基质（ECM）分布进行了半定量评估。在产卵后发育的最初10小时内，在胚盘的透明区和不透明区均发现了纤连蛋白。在透明区，纤连蛋白：（1）与上胚层的基膜相关；（2）存在于上胚层和下胚层细胞之间；（3）偶尔内化于下胚层细胞中。沿着胚胎轴，ECM的短暂高密度与向前快速扩展的下胚层前端相关。在透明区的边缘区域观察到非常高的纤连蛋白密度，此处上胚层和更深层细胞层显示出接触和强烈重排。在不透明区，起初仅在接触细胞之间偶尔发现纤连蛋白，但其密度在发育的第一天稳步且显著增加。针对鸡胚的早期形态发生，讨论了富含纤连蛋白的ECM区域分布的这些快速而显著的变化。
