Obermair Franz-Josef, Fiorelli Roberto, Schroeter Aileen, Beyeler Sarah, Blatti Claudia, Zoerner Bjoern, Thallmair Michaela
Brain Research Institute, Swiss Federal Institute of Technology and University of Zurich, Winterthurerstrasse 190, Zurich, Switzerland.
Stem Cell Res. 2010 Sep;5(2):131-43. doi: 10.1016/j.scr.2010.05.001. Epub 2010 May 16.
Adult neural stem and progenitor cells (NSPCs) are usually defined retrospectively by their ability to proliferate in vivo (bromodeoxyuridine uptake) or to form neurospheres and to differentiate into neurons, astrocytes and oligodendrocytes in vitro. Additional strategies to identify and to isolate NSPCs are of great importance for the investigation of cell differentiation and fate specification. Using the cell surface molecules Prominin-1 and Lewis X and a metabolic marker, the aldehyde dehydrogenase activity, we isolated and characterized five main populations of NSPCs in the neurogenic subventricular zone (SVZ) and the non-neurogenic spinal cord (SC). We used clonal analysis to assess neurosphere formation and multipotency, BrdU retention to investigate in vivo proliferation activity and quantified the expression of NSPC associated genes. Surprisingly, we found many similarities in NSPC subpopulations derived from the SVZ and SC suggesting that subtypes with similar intrinsic potential exist in both regions. The marker defined classification of NSPCs will help to distinguish subpopulations of NSPCs and allows their prospective isolation using fluorescence activated cell sorting.
成体神经干细胞和祖细胞（NSPCs）通常通过其在体内增殖的能力（溴脱氧尿苷摄取）或形成神经球并在体外分化为神经元、星形胶质细胞和少突胶质细胞的能力进行回顾性定义。识别和分离NSPCs的其他策略对于细胞分化和命运决定的研究非常重要。利用细胞表面分子Prominin-1和Lewis X以及一种代谢标志物醛脱氢酶活性，我们在神经发生的脑室下区（SVZ）和非神经发生的脊髓（SC）中分离并鉴定了NSPCs的五个主要群体。我们使用克隆分析来评估神经球的形成和多能性，用BrdU保留来研究体内增殖活性，并对NSPC相关基因的表达进行定量。令人惊讶的是，我们发现源自SVZ和SC的NSPC亚群有许多相似之处，这表明两个区域都存在具有相似内在潜能的亚型。基于标志物定义的NSPC分类将有助于区分NSPC的亚群，并允许使用荧光激活细胞分选对其进行前瞻性分离。
