Department of Food Science, University of Guelph,Guelph, Ontario N1G2W1, Canada.
J Agric Food Chem. 2010 Jul 14;58(13):7624-33. doi: 10.1021/jf1011598.
Long-term oxidative stress in the gastrointestinal tract can lead to the development of chronic intestinal disorders. Many food-derived antioxidants are effective in vitro, but the variable reports of in vivo efficacy and the pro-oxidant nature of some antioxidants necessitate alternative strategies for the reduction of in vivo oxidative stress. Compounds that up-regulate the production of endogenous antioxidants such as glutathione (GSH) and antioxidant enzymes provide novel approaches for the restoration of redox homeostatis. Egg yolk peptides (EYP) prepared from Alcalase and protease N digestion of delipidated egg yolk proteins were found to exhibit antioxidative stress properties. The effect of EYP supplementation was examined in a hydrogen peroxide-induced human colon cell line and in an animal model of intestinal oxidative stress. EYP significantly reduced the pro-inflammatory cytokine, IL-8, in Caco-2 cells. In piglets given intraperitoneal infusions of hydrogen peroxide, EYP treatment increased GSH and gamma-glutamylcysteine synthetase mRNA expression and activity, significantly increased antioxidant enzyme activities, in particular catalase and glutathione S-transferase activities, and reduced protein and lipid oxidation in the duodenum, jejunum, ileum, and colon. Furthermore, EYP boosted the systemic antioxidant status in blood by increasing the GSH concentration in red blood cells. These results suggest that EYP supplementation is a novel strategy for the reduction of intestinal oxidative stress.
长期的胃肠道氧化应激可导致慢性肠道疾病的发生。许多来源于食物的抗氧化剂在体外是有效的，但一些抗氧化剂的体内功效的可变报告和促氧化性质需要替代策略来减少体内氧化应激。上调内源性抗氧化剂（如谷胱甘肽（GSH）和抗氧化酶）产生的化合物为恢复氧化还原内稳态提供了新的方法。用 Alcalase 和蛋白酶 N 消化脱脂蛋白蛋黄蛋白制备的蛋黄肽（EYP）被发现具有抗氧化应激特性。在过氧化氢诱导的人结肠细胞系和肠道氧化应激动物模型中检查了 EYP 的补充效果。EYP 显著降低了 Caco-2 细胞中的促炎细胞因子 IL-8。在接受腹腔内过氧化氢输注的仔猪中，EYP 处理增加了 GSH 和γ-谷氨酰半胱氨酸合成酶 mRNA 表达和活性，显著增加了抗氧化酶活性，特别是过氧化氢酶和谷胱甘肽 S-转移酶活性，并减少了十二指肠、空肠、回肠和结肠中的蛋白质和脂质氧化。此外，EYP 通过增加红细胞中的 GSH 浓度来提高全身抗氧化状态。这些结果表明，EYP 补充是减少肠道氧化应激的一种新策略。
