Department of Pathology, University of Pittsburgh, 200 Lothrop St, Pittsburgh, PA 15213, USA.
J Neuroinflammation. 2010 Jun 11;7:34. doi: 10.1186/1742-2094-7-34.
CHI3L1 (YKL-40) is up-regulated in a variety of inflammatory conditions and cancers. We have previously reported elevated CHI3L1 concentration in the cerebrospinal fluid (CSF) of human and non-human primates with lentiviral encephalitis and using immunohistochemistry showed that CHI3L1 was associated with astrocytes.
In the current study CHI3L1 transcription and expression were evaluated in a variety of acute and chronic human neurological diseases.
ELISA revealed significant elevation of CHI3L1 in the CSF of multiple sclerosis (MS) patients as well as mild elevation with aging. In situ hybridization (ISH) showed CHI3L1 transcription mostly associated with reactive astrocytes, that was more pronounced in inflammatory conditions like lentiviral encephalitis and MS. Comparison of CHI3L1 expression in different stages of brain infarction showed that YKL40 was abundantly expressed in astrocytes during acute phases and diminished to low levels in chronic infarcts.
Taken together, these findings demonstrate that CHI3L1 is induced in astrocytes in a variety of neurological diseases but that it is most abundantly associated with astrocytes in regions of inflammatory cells.
CHI3L1（YKL-40）在多种炎症和癌症中上调。我们之前报道过，在人类和非人类灵长类动物的慢病毒脑炎的脑脊液（CSF）中，CHI3L1 浓度升高，并用免疫组织化学显示 CHI3L1 与星形胶质细胞有关。
在本研究中，我们评估了 CHI3L1 在多种急性和慢性人类神经疾病中的转录和表达。
ELISA 显示，多发性硬化症（MS）患者的 CSF 中 CHI3L1 显著升高，随着年龄的增长也有轻度升高。原位杂交（ISH）显示 CHI3L1 转录主要与反应性星形胶质细胞有关，在慢病毒脑炎和 MS 等炎症状态下更为明显。比较脑梗死不同阶段的 CHI3L1 表达显示，YKL40 在急性阶段在星形胶质细胞中大量表达，在慢性梗死中减少到低水平。
综上所述，这些发现表明，CHI3L1 在多种神经疾病中星形胶质细胞中诱导，但在炎症细胞区域与星形胶质细胞最密切相关。
