College of Animal Science and Veterinary Medicine, JiLin University, 5333 Xi 'an Road, Changchun 130062, China.
Virol J. 2010 Jun 11;7:124. doi: 10.1186/1743-422X-7-124.
Mink enteritis virus (MEV) causes a highly contagious viral disease of mink with a worldwide distribution. MEV has a linear, single-stranded, negative-sense DNA with a genome length of approximately 5,000 bp. The VP2 protein is the major structural protein of the parvovirus encoded by the vp2 gene. VP2 is highly antigenic and plays important roles in determining viral host ranges and tissue tropisms. This study describes the bionomics and vp2 gene analysis of a mutated strain, MEV-DL, which was isolated recently in China and outlines its homologous relationships with other selected strains registered in Genbank.
The MEV-DL strain can infect F81 cells with cytopathic effects. Pig erythrocytes were agglutinated by the MEV-DL strain. The generation of MEV-DL in F81 cells could infect mink within three months and cause a disease that was similar to that caused by wild-type MEV. A comparative analysis of the vp2 gene nucleotide (nt) sequence of MEV-DL showed that this was more than 99% homologous with other mink enteritis parvoviruses in Genbank. However, the nucleotide residues at positions 1,065 and 1,238 in the MEV-DL strain of the vp2 gene differed from those of all the other MEV strains described previously. It is noteworthy that the mutation at the nucleotide residues position 1,238 led to Asp/Gly replacement. This may lead to structural changes. A phylogenetic tree and sequence distance table were obtained, which showed that the MEV-DL and ZYL-1 strains had the closest inheritance distance.
A new variation of the vp2 gene exists in the MEV-DL strain, which may lead to structural changes of the VP2 protein. Phylogenetic analysis showed that MEV-DL may originate from the ZYL-1 strain in DaLian.
水貂肠炎病毒（MEV）引起一种具有全球分布的水貂高度传染性病毒性疾病。MEV 具有线性、单链、负义 DNA，基因组长度约为 5000bp。VP2 蛋白是 parvovirus 编码的主要结构蛋白，由 vp2 基因编码。VP2 高度抗原性，在决定病毒宿主范围和组织嗜性方面发挥重要作用。本研究描述了最近在中国分离到的一种突变株 MEV-DL 的生物学特性和 vp2 基因分析，并概述了其与 Genbank 中登记的其他选定株的同源关系。
MEV-DL 株可感染 F81 细胞并产生细胞病变效应。猪红细胞被 MEV-DL 株凝集。MEV-DL 在 F81 细胞中的生成可在三个月内感染水貂，并引起与野生型 MEV 相似的疾病。MEV-DL 的 vp2 基因核苷酸（nt）序列的比较分析表明，它与 Genbank 中其他水貂肠炎细小病毒高度同源，超过 99%。然而，vp2 基因 MEV-DL 株的核苷酸位置 1065 和 1238 处的核苷酸残基与所有先前描述的其他 MEV 株不同。值得注意的是，vp2 基因位置 1238 处的核苷酸残基突变导致天冬氨酸/甘氨酸取代。这可能导致结构发生变化。获得了系统发育树和序列距离表，表明 MEV-DL 和 ZYL-1 株具有最接近的遗传距离。
MEV-DL 株的 vp2 基因存在新的变异，可能导致 VP2 蛋白的结构发生变化。系统发育分析表明，MEV-DL 可能起源于大连的 ZYL-1 株。
