State Key Laboratory of Agrobiotechnology, Department of Biochemistry and Molecular Biology, College of Biological Sciences, China Agricultural University, Beijing, China.
PLoS One. 2013 Dec 11;8(12):e81515. doi: 10.1371/journal.pone.0081515. eCollection 2013.
Mink enteritis virus (MEV) is one of the most important viral pathogens in the mink industry. Recent studies have showed that microRNAs (miRNAs), small noncoding RNAs of length ranging from 18-23 nucleotides (nt) participate in host-pathogen interaction networks; however, whether or not miRNAs are involved in MEV infection has not been reported. Our study revealed that miRNA miR-181b inhibited replication of MEV in the feline kidney (F81) cell line by targeting the MEV non-structural protein 1 (NS1) messenger RNA (mRNA) coding region, resulting in NS1 translational repression, while MEV infection reduced miR-181b expression. This is the first description of cellular miRNAs modulating MEV infection in F81 cells, providing further insight into the mechanisms of viral infection, and may be useful in development of naturally-occurring miRNAs antiviral strategies.
水貂肠炎病毒（MEV）是水貂养殖业中最重要的病毒病原体之一。最近的研究表明，微小 RNA（miRNA），即长度在 18-23 个核苷酸（nt）范围内的小非编码 RNA，参与宿主-病原体相互作用网络；然而，miRNA 是否参与 MEV 感染尚未见报道。本研究表明，miRNA miR-181b 通过靶向 MEV 非结构蛋白 1（NS1）信使 RNA（mRNA）编码区抑制猫肾（F81）细胞系中的 MEV 复制，导致 NS1 翻译抑制，而 MEV 感染降低了 miR-181b 的表达。这是首次描述细胞 miRNA 调节 F81 细胞中 MEV 感染的情况，为病毒感染的机制提供了进一步的认识，并且可能有助于开发天然存在的 miRNA 抗病毒策略。
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