枸橼酸西地那非是否会影响体外硝苯地平对子宫肌收缩反应的影响？
Does sildenafil citrate affect myometrial contractile response to nifedipine in vitro?
机构信息
Department of Obstetrics and Gynecology, University of Texas Medical Branch, Galveston, TX, USA.
出版信息
Am J Obstet Gynecol. 2010 Sep;203(3):252.e1-5. doi: 10.1016/j.ajog.2010.05.007. Epub 2010 Jun 11.
OBJECTIVE
We sought to test the hypothesis that sildenafil citrate (SC) at low concentrations potentiates the tocolytic effects of nifedipine in vitro.
STUDY DESIGN
Myometrial biopsies were obtained from 22 term nonlaboring women undergoing scheduled cesarean delivery. Tissue strips were suspended in organ chambers for isometric tension recording, and incubated for 30 minutes with either SC at 231 ng/mL or solvent. The effects of cumulative doses (10(-10) to 10(-5) mol/L) of nifedipine on spontaneous and oxytocin-induced uterine contractility were then determined. Areas under the contraction curve were compared using 1-way analysis of variance with Tukey post hoc test (significance: P < .05).
RESULTS
Nifedipine significantly inhibited spontaneous and oxytocin-induced myometrial contractility. Preincubation with SC increased response to nifedipine and significantly potentiated its inhibitory effect at 10(-8) mol/L, without affecting oxytocin-induced contractile response.
CONCLUSION
At concentrations within a therapeutic window, SC increases myometrial sensitivity to nifedipine.
目的
我们旨在验证以下假说，即在低浓度下，枸橼酸西地那非（SC）可增强硝苯地平的体外保胎作用。
研究设计
我们从 22 名处于足月非临产状态并计划行剖宫产的孕妇中获取子宫肌活检标本。将组织条悬挂在器官室中进行等长张力记录，并在含有 231ng/ml SC 或溶剂的溶液中孵育 30 分钟。然后，确定累积剂量（10（-10）至 10（-5）mol/L）的硝苯地平对自发性和催产素诱导的子宫收缩的影响。使用单向方差分析和 Tukey 事后检验比较收缩曲线下面积（显著性：P<.05）。
结果
硝苯地平显著抑制自发性和催产素诱导的子宫肌收缩。SC 的预孵育增加了对硝苯地平的反应，并在 10（-8）mol/L 时显著增强其抑制作用，而不影响催产素诱导的收缩反应。
结论
在治疗窗浓度内，SC 可增加子宫对硝苯地平的敏感性。
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