利用糖蜜提高酵母细胞的脂类积累特性，用于生物柴油生产。
Improving the lipid accumulation properties of the yeast cells for biodiesel production using molasses.
机构信息
Department of Biology, Faculty of Science, Ankara University, 06100 Beşevler, Ankara, Turkey.
出版信息
Bioresour Technol. 2010 Oct;101(20):7988-90. doi: 10.1016/j.biortech.2010.05.054. Epub 2010 Jun 12.
In this article the high lipid accumulation potential of Candida lipolytica, Candida tropicalis and Rhodotorula mucilaginosa cells were shown in molasses medium. Four different pH values were examined (4-7) to discover the optimum lipid accumulation medium. Varied concentrations of (NH₄)₂SO₄ (0.5, 1.0, 1.5 g/L) and molasses (6%, 8%, 10%) were tested to find the optimum carbon and nitrogen amounts for the highest cellular lipid production. It is shown that the maximum lipid content could be achieved in the medium containing 8% molasses solution and 1.0 g/L (NH₄)₂SO₄ at pH 5 after four days of incubation time. The maximum lipid contents and methyl ester yields were measured as 59.9% and 84.9% for C. lipolytica, 46.8% and 93.2% for C. tropicalis, 69.5% and 92.3% for R. mucilaginosa. Because of the dominant fatty acids were C16 and C18 in the lipids of yeast cells, these crude lipids could be promising feedstock for biodiesel production.
本文展示了 Candida lipolytica、Candida tropicalis 和 Rhodotorula mucilaginosa 细胞在糖蜜培养基中的高脂质积累潜力。研究了四种不同的 pH 值（4-7），以发现最佳的脂质积累培养基。测试了不同浓度的(NH₄)₂SO₄（0.5、1.0、1.5 g/L）和糖蜜（6%、8%、10%），以找到最高细胞脂质产量的最佳碳氮比。结果表明，在 pH 值为 5、含 8%糖蜜溶液和 1.0 g/L(NH₄)₂SO₄的培养基中，经过四天的培养时间，可达到最大的脂质含量。最大脂质含量和甲酯产率分别为 59.9%和 84.9%的 C. lipolytica、46.8%和 93.2%的 C. tropicalis、69.5%和 92.3%的 R. mucilaginosa。由于酵母细胞脂质中的优势脂肪酸为 C16 和 C18，这些粗脂质有望成为生物柴油生产的原料。
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