Department of Food Chemistry and Biocatalysis, Wrocław University of Environmental and Life Sciences, Norwida 25, 50-375 Wrocław, Poland.
Molecules. 2023 Jul 15;28(14):5423. doi: 10.3390/molecules28145423.
Currently, there is an increasing number of cases of fungal infections caused by opportunistic strains of the yeast , mainly in immunocompromised patients during hospitalization. The excessive use of antibiotics and azole compounds increases the risk of resistance to microorganisms. A new alternative to these drugs may be synthetic phthalide lactones with a structure identical to or similar to the natural ones found in celery plants, which show low toxicity and relatively high fungistatic activity. In the present study, the fungistatic activity of seven phthalide lactones was determined against IHEM 18459. We showed that 3--butylidenephthalide, the most potent compound selected in the microdilution test, caused a dose-dependent decrease in dry yeast biomass. Phthalide accumulated in yeast cells and contributed to an increase in reactive oxygen species content. The synergistic effect of fluconazole resulted in a reduction in the azole concentration required for yeast inhibition. We observed changes in the color of the yeast cultures; thus, we conducted experiments to prove that the carotenoid profile was altered. The addition of lactones also triggered a decline in fatty acid methyl esters.
目前，由酵母中的机会性菌株引起的真菌感染病例越来越多，主要发生在住院期间免疫功能低下的患者中。抗生素和唑类化合物的过度使用增加了微生物耐药的风险。这些药物的一种新替代品可能是合成苯酞内酯，其结构与芹菜植物中发现的天然苯酞内酯相同或相似，显示出低毒性和相对较高的抑菌活性。在本研究中，测定了七种苯酞内酯对 IHEM 18459 的抑菌活性。我们表明，在所选择的微量稀释试验中最有效的化合物 3--丁烯基苯酞导致干酵母生物量呈剂量依赖性下降。苯酞积累在酵母细胞中，并导致活性氧含量增加。氟康唑的协同作用导致抑制酵母所需的唑类浓度降低。我们观察到酵母培养物颜色的变化；因此，我们进行了实验以证明类胡萝卜素谱发生了改变。内酯的添加也引发了脂肪酸甲酯的下降。
