通过抑制凝血酶活性实现凝血酶底物固定化聚合物表面的体外非血栓形成性

In vitro non-thrombogenicity of a thrombin-substrate-immobilized polymer surface by the inhibition of thrombin activity.

作者信息

Ito Y, Liu L S, Imanishi Y

机构信息

Department of Polymer Chemistry, Kyoto University, Japan.

出版信息

J Biomater Sci Polym Ed. 1991;2(2):123-38. doi: 10.1163/156856291x00115.

DOI:10.1163/156856291x00115

PMID:2054331

Abstract

Derivatives of thrombin substrate were synthesized and immobilized on a poly(acrylic acid)-grafted polyurethane film. The carboxyl terminal of the thrombin substrate peptide should be blocked for a higher inhibitory effect of the thrombin activity. Immobilization of the thrombin substrate peptide enhanced adsorption and inactivation of thrombin on the polymer film to prolong the time for fibrin network formation, and suppressed adhesion and deformation of platelets on the film. Consequently, in vitro thrombus formation on the polymer film was strongly suppressed.

摘要

合成了凝血酶底物衍生物并将其固定在聚丙烯酸接枝的聚氨酯薄膜上。为了对凝血酶活性产生更高的抑制作用，凝血酶底物肽的羧基末端应被封闭。凝血酶底物肽的固定增强了凝血酶在聚合物薄膜上的吸附和失活，从而延长了纤维蛋白网络形成的时间，并抑制了血小板在薄膜上的黏附和变形。因此，聚合物薄膜上的体外血栓形成受到了强烈抑制。