Institute of Microelectronics, Peking University, Beijing, PR China.
Lab Chip. 2010 Sep 21;10(18):2338-40. doi: 10.1039/c004758a. Epub 2010 Jun 14.
Here we report an approach utilizing a suspended nanoparticle crystal (S-NPC) as an electrical read-out biosensor based on a nanofluidic electrokinetics principle. As a preliminary demonstration, streptavidin-modified S-NPC with a particle diameter of 520 nm was used to detect biotin in a PBS buffer. The present result indicated that the detection range of biotin by this nanofluidics-based biosensor was about 1 nM-10 muM (in 10(-4)x PBS) with a sensitivity of 160 nS/nM. Being easy to get established, low-cost, and having large electrical read-out signal, the present S-NPC is believed to be a promising biosensing scheme in the micro total analysis system.
在这里，我们报告了一种利用悬浮纳米颗粒晶体（S-NPC）作为电读出生物传感器的方法，该传感器基于纳流动力学原理。作为初步演示，我们使用直径为 520nm 的链霉亲和素修饰的 S-NPC 来检测 PBS 缓冲液中的生物素。本研究结果表明，这种基于纳流控的生物传感器检测生物素的范围约为 1nM-10μM（在 10(-4)x PBS 中），灵敏度为 160nS/nM。由于易于建立、成本低且具有较大的电读出信号，因此本研究中的 S-NPC 被认为是微全分析系统中一种很有前途的生物传感方案。
