Christoffersen S, Serra I, Terreni M, Piskur J
Department of Cell and Organism Biology, Lund University, Lund, Sweden.
Nucleosides Nucleotides Nucleic Acids. 2010 Jun;29(4-6):445-8. doi: 10.1080/15257771003741422.
Four Clostridium perfringens phosphorylases were subcloned, overexpressed and analyzed for their substrate specificity. DeoD(1) and PunA could use a variety of purine substrates, including an antiviral drug 2',3'-dideoxyinosine (ddI). In one-pot synthesis using Clostridium phosphorylases, 2',3'-dideoxyuridine and hypoxanthine were converted to ddI at yield of about 30%.
对四种产气荚膜梭菌磷酸化酶进行了亚克隆、过表达，并分析了它们的底物特异性。DeoD(1)和PunA可以利用多种嘌呤底物，包括抗病毒药物2',3'-双脱氧肌苷（ddI）。在使用梭菌磷酸化酶的一锅法合成中，2',3'-双脱氧尿苷和次黄嘌呤以约30%的产率转化为ddI。
Zotero 插件