Bioprocess Engineering, Department of Biotechnology, TU Berlin, Ackerstr. 76, 13355 Berlin, Germany.
Bioprocess Engineering, Department of Biotechnology, TU Berlin, Ackerstr. 76, 13355 Berlin, Germany; BioNukleo GmbH, Ackerstr. 76, 13355 Berlin, Germany.
Biochim Biophys Acta Proteins Proteom. 2020 Feb;1868(2):140304. doi: 10.1016/j.bbapap.2019.140304. Epub 2019 Nov 2.
Nucleoside phosphorylases catalyze the reversible phosphorolysis of pyrimidine and purine nucleosides in the presence of phosphate. They are valuable catalysts in the synthesis of nucleosides and their analogues, which are often used as pharmaceuticals or their precursors. Thermostable nucleoside phosphorylases are promising biocatalysts, as they withstand harsh reaction conditions such as high pH or the addition of organic solvents. In this review, the characteristics and properties of thermostable nucleoside phosphorylases are described. Differences in amino acid content and protein structure were compared to their mesophilic homologues to identify features involved in thermostability. Substrate spectra of thermostable nucleoside phosphorylases were analyzed, and it is shown that thermostable nucleoside phosphorylases have a wider substrate spectrum than their mesophilic counterparts. Thus, thermostable nucleoside phosphorylases are interesting biocatalysts for industrial applications.
核苷磷酸化酶在存在磷酸盐的情况下催化嘧啶和嘌呤核苷的可逆磷酸解。它们是核苷及其类似物合成的有价值的催化剂，核苷及其类似物通常用作药物或其前体。热稳定的核苷磷酸化酶是很有前途的生物催化剂，因为它们能够承受苛刻的反应条件，如高 pH 值或有机溶剂的添加。在这篇综述中，描述了热稳定的核苷磷酸化酶的特性和性质。比较了氨基酸含量和蛋白质结构的差异与其嗜温同系物，以确定与耐热性相关的特征。分析了热稳定的核苷磷酸化酶的底物谱，结果表明热稳定的核苷磷酸化酶的底物谱比其嗜温对应物更宽。因此，热稳定的核苷磷酸化酶是工业应用中有趣的生物催化剂。
