George M. Leader Family Laboratory, Department of Neurosurgery, Pennsylvania State University College of Medicine, 500 University Drive (H110), Hershey, PA 17033-0850, USA.
Muscle Nerve. 2010 Jul;42(1):95-103. doi: 10.1002/mus.21625.
Amyotrophic lateral sclerosis (ALS) is a progressive neurodegenerative disease with complicated pathogenesis with variable presentation and disease progression. There is a critical need for a panel of biomarkers to provide clinicians and researchers with additional information. In this study, multiplex immunoassays were used to screen a number of cytokines, growth factors, and iron-related proteins. ALS patients had significantly higher plasma levels of L-ferritin and lower concentrations of transferrin when compared to healthy controls and together classified a test group of subjects with 82% accuracy. Duration of ALS symptoms correlated positively with levels of monocyte chemoattractant protein 1 (MCP-1) and negatively with levels of granulocyte-macrophage colony stimulating factor (GM-CSF). The biomarker profile suggests iron homeostasis is disrupted in ALS patients, and changes in ferritin and transferrin (Tf) appear to be indicators of ongoing inflammatory processes. The data demonstrate a plasma biomarker profile in ALS patients that may differ from published reports of cerebrospinal fluid biomarkers.
肌萎缩侧索硬化症（ALS）是一种具有复杂发病机制的进行性神经退行性疾病，其表现和疾病进展存在差异。因此，迫切需要一组生物标志物，为临床医生和研究人员提供更多信息。在这项研究中，使用多重免疫分析筛选了多种细胞因子、生长因子和与铁相关的蛋白质。与健康对照组相比，ALS 患者的血浆 L 型铁蛋白水平显著升高，转铁蛋白浓度降低，联合检测可将实验组受试者准确分类 82%。ALS 症状的持续时间与单核细胞趋化蛋白 1（MCP-1）水平呈正相关，与粒细胞-巨噬细胞集落刺激因子（GM-CSF）水平呈负相关。该生物标志物谱表明 ALS 患者的铁稳态受到破坏，铁蛋白和转铁蛋白（Tf）的变化似乎是正在进行的炎症过程的指标。这些数据显示了 ALS 患者的血浆生物标志物谱，与已发表的脑脊液生物标志物报告可能有所不同。
