嵌合型溶瘤腺病毒SG235对白血病细胞的体外抑制作用

[Inhibitive effects of chimeric oncolytic adenovirus SG235 on leukemia cells in vitro].

作者信息

Yang Chun-mei, Liu Hui, Yang Xiu-di, Jin Jie, Qian Wen-bin

机构信息

The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310003, China.

出版信息

Zhejiang Da Xue Xue Bao Yi Xue Ban. 2010 May;39(3):226-30. doi: 10.3785/j.issn.1008-9292.2010.03.002.

DOI:10.3785/j.issn.1008-9292.2010.03.002

PMID:20544981

Abstract

OBJECTIVE

To investigate the inhibitive effects of chimeric oncolytic adenovirus SG235 on leukemia cells in vitro.

METHODS

The ability of SG235 to infect leukemia cells and the expression of CD46 on blasts from the patient with leukemia were detected by flow cytometry (FACS). The cytotoxicity of the virus was evaluated by MTT assay. Apoptosis induced by SG235 was detected with Annexin-V/PI staining and TUNEL assay followed by FACS analysis.

RESULT

The majority of leukemia cells from the patient with acute leukemia was CD46-positive. GFP-positive cells were 45.1%, 35.7%, 54.2%, 37.0%, 30.1%, %67.1, 17.2% and 33.1% in Mutz-1, Kasumi-1, K562, HL60, Molt- 4, RPMI8226, L428, and Jurkat cell lines treated with SG235-EGFP vector at MOI (multiplicity of infection) of 50 for 48 h.SG235 treatment resulted in marked growth inhibition and apoptosis of Kassumi-1 cells, and also significantly inhibited expression of p-Akt.

CONCLUSION

The chimeric oncolytic adenovirus SG235 can infect leukemia cell effectively and results in the growth inhibition and apoptosis of Kasumi-1 cells in vitro.

摘要

目的

研究嵌合型溶瘤腺病毒SG235对白血病细胞的体外抑制作用。

方法

采用流式细胞术（FACS）检测SG235感染白血病细胞的能力及白血病患者原始细胞上CD46的表达。采用MTT法评估病毒的细胞毒性。用膜联蛋白-V/碘化丙啶染色及TUNEL法检测SG235诱导的凋亡，随后进行FACS分析。

结果

急性白血病患者的大多数白血病细胞CD46呈阳性。在感染复数（MOI）为50的情况下，用SG235-EGFP载体处理Mutz-1、Kasumi-1、K562、HL60、Molt-4、RPMI8226、L428和Jurkat细胞系48小时后，绿色荧光蛋白（GFP）阳性细胞分别为45.1%、35.7%、54.2%、37.0%、30.1%、67.1%、17.2%和33.1%。SG235处理导致Kasumi-1细胞明显生长抑制和凋亡，且显著抑制p-Akt的表达。