[黄芪的快速分辨率液相色谱-紫外-质谱指纹图谱]
[RRLC-UV-MS fingerprint of Radix Astragali].
作者信息
Su Xian, Wu Lijun, Tu Pengfei
机构信息
Department of Traditional Chinese Medicine & Pharmacognosy, Shenyang Pharmaceutical University, Shenyang 110016, China.
出版信息
Zhongguo Zhong Yao Za Zhi. 2010 Mar;35(6):732-5. doi: 10.4268/cjcmm20100617.
OBJECTIVE
To establish a method for detecting the fingerprint of Radix Astragali by RRLC-UV-MS.
METHOD
Separation was performed on an Agilent Zorbax Extend C18 column (2.1 mm x 100 mm, 1.8 microm). Gradient elution was performed by the mobile phases consisting of acetonitrile and 0.1% formic acid with the flow rate of 0.3 mL x min(-1), the detection wavelength was set at 254 nm, and the column temperature was 30 degrees C.
RESULT
The method of fingerprint analysis on Radix Astragali by RRLC-UV-MS was established. Eleven samples of Radix Astragali were analyzed, the similarities were over 0.92, and seven peaks in the fingerprint were designated.
CONCLUSION
The method can be applied to the quality control and studies on chemical constituents of Radix Astragali.
目的
建立一种采用快速液相色谱-紫外-质谱联用（RRLC-UV-MS）检测黄芪指纹图谱的方法。
方法
采用安捷伦Zorbax Extend C18柱（2.1 mm×100 mm，1.8μm）进行分离。流动相为乙腈和0.1%甲酸，进行梯度洗脱，流速为0.3 mL·min⁻¹，检测波长设定为254 nm，柱温为30℃。
结果
建立了黄芪RRLC-UV-MS指纹图谱分析方法。对11个黄芪样品进行分析，相似度均超过0.92，并标定了指纹图谱中的7个峰。
结论
该方法可用于黄芪的质量控制和化学成分研究。
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