Mahdavi M, Ebtekar M, Azadmanesh K, Khorramkhorshid H R, Rahbarizadeh F, Yazdi M H, Zabihollahi R, Abolhassani M, Hassan Z M
Department of Immunology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
Acta Virol. 2010;54(2):131-6. doi: 10.4149/av_2010_02_131.
Many Human immunodeficiency virus (HIV) candidate vaccines have been tested in clinical trials, but none was sufficiently effective in the prevention of HIV infection. A HIV vaccine should induce humoral as well as cell-mediated response, the latter including the cytotoxic CD8+ T lymphocyte (CTL) response. In this study, we immunized BALB/c mice with a purified fusion peptide Gag p24-Nef and evaluated immune responses. As for the cellular responses, the adjuvanted fusion peptide induced lymphocyte proliferation, CTL response, and cytokines IFN-gamma and IL-4 in the Th1 pattern. Humoral immune response to the adjuvanted fusion peptide included an increase in IgG antibodies of more IgG2a than IgG1 subtype. These results indicate that the employed HIV-1 peptide construct can elicit both cellular and humoral immune responses in mice. Further studies aimed at memory T cells and other aspects of immune responses are needed before a comprehensive assessment of this candidate vaccine could be provided.
epitopes; fusion peptide; HIV-1 p24-Nef; immune response.
许多人类免疫缺陷病毒（HIV）候选疫苗已在临床试验中进行了测试，但没有一种在预防HIV感染方面足够有效。HIV疫苗应诱导体液免疫和细胞介导的免疫反应，后者包括细胞毒性CD8 + T淋巴细胞（CTL）反应。在本研究中，我们用纯化的融合肽Gag p24-Nef免疫BALB / c小鼠并评估免疫反应。至于细胞反应，佐剂化融合肽诱导淋巴细胞增殖、CTL反应以及Th1模式的细胞因子IFN-γ和IL-4。对佐剂化融合肽的体液免疫反应包括IgG抗体增加，其中IgG2a亚型比IgG1亚型更多。这些结果表明，所使用的HIV-1肽构建体可在小鼠中引发细胞免疫和体液免疫反应。在对该候选疫苗进行全面评估之前，需要针对记忆T细胞和免疫反应的其他方面进行进一步研究。
表位；融合肽；HIV-1 p24-Nef；免疫反应。
