Components R&D group, National Science Laboratory, Scottish National Blood Transfusion Service, Edinburgh, UK.
Vox Sang. 2010 Nov;99(4):362-8. doi: 10.1111/j.1423-0410.2010.01355.x.
The DiaMed Impact R tests platelet function under close to physiological flow conditions. The machine is designed to use whole blood but by adding back compatible red cells, it can be used to study stored platelet concentrates. To date, red cells ≤14 days old have been used. In this study, the effect on the assay of using red cells stored for up to 60 days was examined.
This study looked at buffy coat-derived platelet concentrates on day 2 of storage along with various stored red-blood-cells (RBC). To determine whether the age of the RBC is a factor in supporting adhesion and aggregation, platelets were assayed with either RBC stored between 2 and 60 days or with separated 'young' and 'old' red cell populations obtained using a centrifugation method and confirmed by percoll gradient analysis.
A statistically significant difference was observed between red-blood-cells stored for ≤20 days compared with those which have been stored for 21-60 days in respect of their ability to support platelet adhesion (SC) and aggregation (AS) (P<0·01). Separating red cells by centrifugation into top (young population) and bottom (old population) showed that the effect of storage was much greater than was any difference between young and old at the individual time-points e.g. 'young' red cells from stored units were poorer at supporting platelet adhesion and aggregation than 'young' red cells from fresh units.
Results suggest that the red cells should be stored for less than 21 days when using this assay. This assay may also allow assessment of red cell functionality.
DiaMed Impact R 测试在接近生理流动条件下的血小板功能。该机器旨在使用全血，但通过添加回兼容的红细胞，可以用于研究储存的血小板浓缩物。迄今为止，使用的红细胞年龄不超过 14 天。在这项研究中，研究了使用储存长达 60 天的红细胞对检测的影响。
本研究观察了储存第 2 天的浓缩血小板与各种储存的红细胞（RBC）。为了确定 RBC 的年龄是否是支持粘附和聚集的因素，使用储存 2 至 60 天的 RBC 或使用离心法获得的分离的“年轻”和“年老”红细胞群体来检测血小板，并用 Percoll 梯度分析进行确认。
与储存 21-60 天的 RBC 相比，储存≤20 天的 RBC 在支持血小板粘附（SC）和聚集（AS）方面存在显著差异（P<0·01）。通过离心将红细胞分离为顶部（年轻群体）和底部（年老群体），结果表明储存的影响比单个时间点之间的年轻和年老之间的差异更大，例如，来自储存单位的“年轻”红细胞在支持血小板粘附和聚集方面的能力比来自新鲜单位的“年轻”红细胞差。
结果表明，在使用该检测时，红细胞的储存时间应少于 21 天。该检测还可以评估红细胞的功能。
