Kellstein D E, Price D D, Mayer D J
Department of Physiology, Medical College of Virginia, Virginia Commonwealth University, Richmond 23298.
Brain Res. 1991 Feb 1;540(1-2):302-6. doi: 10.1016/0006-8993(91)90524-y.
Extracellular single unit recordings were made from dorsal horn nociceptive neurons of intact, urethane-anesthetized rats during controlled electrical stimulation of the hind paw. Neither local superfusion of cholecystokinin octapeptide (CCK; 6.4 pmol to 20 nmol) nor the CCK antagonist lorglumide (LGM; 145 fmol to 145 pmol) significantly altered A- or C-fiber evoked firing or spontaneous activity. Pretreatment with CCK, however, significantly attenuated, whereas LGM enhanced, morphine-induced inhibition of C-evoked firing. These findings provide further evidence that CCK functions as a selective antagonist of opioid-induced analgesia.
在对完整的、氨基甲酸乙酯麻醉的大鼠后爪进行控制性电刺激期间，从其背角伤害性神经元进行细胞外单单位记录。胆囊收缩素八肽（CCK；6.4皮摩尔至20纳摩尔）的局部灌注以及CCK拮抗剂洛谷胺（LGM；145飞摩尔至145皮摩尔）均未显著改变A纤维或C纤维诱发的放电或自发活动。然而，CCK预处理显著减弱了吗啡诱导的对C纤维诱发放电的抑制，而LGM则增强了这种抑制。这些发现进一步证明CCK作为阿片类药物诱导镇痛的选择性拮抗剂发挥作用。
