School of Biological Sciences, University of Sydney, New South Wales, Australia.
Plasmid. 2010 Nov;64(3):135-42. doi: 10.1016/j.plasmid.2010.06.001. Epub 2010 Jun 12.
The Staphylococcus aureus multiresistance plasmid pSK1 is the prototype of a family of structurally related plasmids that were first identified in epidemic S. aureus strains isolated in Australia during the 1980s and subsequently in Europe. Here we present the complete 28.15kb nucleotide sequence of pSK1 and discuss the genetic content and evolution of the 14kb region that is conserved throughout the pSK1 plasmid family. In addition to the previously characterized plasmid maintenance functions, this backbone region encodes 12 putative gene products, including a lipoprotein, teichoic acid translocation permease, cell wall anchored surface protein and an Fst-like toxin as part of a Type I toxin-antitoxin system. Furthermore, transcriptional profiling has revealed that plasmid carriage most likely has a minimal impact on the host, a factor that may contribute to the ability of pSK1 family plasmids to carry multiple resistance determinants.
金黄色葡萄球菌多耐药质粒 pSK1 是一组结构相关质粒的原型，这些质粒最初是在 20 世纪 80 年代在澳大利亚分离的流行金黄色葡萄球菌菌株中发现的，随后在欧洲也发现了这些质粒。在这里，我们呈现了 pSK1 的完整 28.15kb 核苷酸序列，并讨论了整个 pSK1 质粒家族中保守的 14kb 区域的遗传内容和进化。除了先前表征的质粒维持功能外，这个骨干区域还编码了 12 个推定的基因产物，包括脂蛋白、磷壁酸转运渗透酶、细胞壁锚定表面蛋白和 Fst 样毒素，作为 I 型毒素-抗毒素系统的一部分。此外，转录谱分析表明，质粒携带对宿主的影响可能很小，这一因素可能有助于 pSK1 家族质粒携带多种耐药决定因子。
