Institute of Bioscience, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
J Biosci Bioeng. 2010 Sep;110(3):338-44. doi: 10.1016/j.jbiosc.2010.02.017. Epub 2010 Mar 29.
Serum deprivation inhibits cell growth and initiates apoptosis cell death in mammalian cell cultures. Since apoptosis is a genetically controlled cell death pathway, over-expression of anti-apoptotic proteins may provide a way to delay apoptosis. This study investigated the ability of the X-linked inhibitor of apoptosis protein (XIAP) to inhibit apoptosis induced by serum deprivation. Study includes evaluation of the ability of XIAP to prolong culture period and its effect on cell proliferation in serum-deprived media. The full length human XIAP was introduced into CHO-K1 cell lines and the effects of XIAP over-expression on the inhibition of apoptosis induced by serum-deprived conditions were examined. In batch cultures, cells over-expressing XIAP showed decreased levels of apoptosis and a higher number of viable cell under serum-deprived conditions compared to the control cell lines. The viability of control cells dropped to 40% after 2days of serum deprivation, the XIAP expressing cells still maintained at a viability higher than 90%. Further investigation revealed that the caspase-3 activity of the CHO-K1 cell line was inhibited as a result of XIAP expression.
血清剥夺抑制哺乳动物细胞培养物中的细胞生长并引发细胞凋亡。由于细胞凋亡是一种受基因控制的细胞死亡途径，因此过表达抗凋亡蛋白可能提供一种延迟细胞凋亡的方法。本研究探讨了 X 连锁凋亡抑制蛋白 (XIAP) 抑制血清剥夺诱导的细胞凋亡的能力。该研究包括评估 XIAP 延长培养时间的能力及其在无血清培养基中对细胞增殖的影响。全长人 XIAP 被引入 CHO-K1 细胞系，研究了 XIAP 过表达对血清剥夺条件下诱导的细胞凋亡的抑制作用。在分批培养中，与对照细胞系相比，过表达 XIAP 的细胞在血清剥夺条件下显示出较低水平的凋亡和更高数量的存活细胞。对照细胞在血清剥夺 2 天后，其存活率降至 40%，而表达 XIAP 的细胞的存活率仍保持在 90%以上。进一步的研究表明，CHO-K1 细胞系中的 caspase-3 活性由于 XIAP 的表达而受到抑制。
