经心脏左心室注射胶原酶后分离和鉴定鼠肝细胞。
Isolation and characterization of murine hepatocytes following collagenase infusion into left ventricle of heart.
机构信息
Program in Tissue Engineering, Department of Parasitology, Nara Medical University, 840 Shijo-cho, Kashihara, Nara 634-8521, Japan.
出版信息
J Biosci Bioeng. 2010 Oct;110(4):487-90. doi: 10.1016/j.jbiosc.2010.04.015. Epub 2010 May 31.
PMID:20547368
Abstract
A method for obtaining mouse hepatocytes by infusing collagenase solution into the left ventricle was established. This technique was shown to be equivalent to the intra-portal infusion method and more practical, especially in postnatal mice with a small body size.
摘要
建立了一种通过向左心室输注胶原酶溶液来获取小鼠肝细胞的方法。该技术与门静脉内输注法等效，并且更实用，尤其是在体型较小的新生小鼠中。
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