Quiroga Lorena P, Lansingh Van, Laspina Florentina, Samudio Margarita, Stanley Jenny, Kaspar Herminia Miño de, Cibils Diógenes, Cibils Pablo
Instituto de Previsión Social, Fundacion Vision e Central Hospital, Asunción, Paraguay.
Arq Bras Oftalmol. 2010 Mar-Apr;73(2):125-8. doi: 10.1590/s0004-27492010000200005.
To determine the conjunctival bacterial flora in cataract surgery patients and the effect of presurgical irrigation with 5% povidone-iodine solution.
Three samples from the inferior conjunctival fornix of the eye to be operated were obtained at baseline before washing (T0) with 10 ml 5% povidone-iodine solution, immediately after washing (T1), and upon completion of surgery (T2). Bacteria from the samples were inoculated in blood agar, chocolate agar, and thioglycolate broth.
A total of 221 patients (n=224 eyes) with a mean age of 67 +/- 13 years were included in the study. Bacteria from the (T0) samples were successfully grown in 73.2% of the thioglycolate agars, 21.0% of the blood agars, and 19.2% of the chocolate agars. Compared with T1 samples, the use of povidone-iodine wash translated into a 60.8% reduction (from 73.2% to 12.5%) in colonization of the conjunctiva (p<0.001), while no significant difference was found between the T1 and T2 samples.
Conjunctival irrigation with a 5% povidone-iodine solution effectively reduces conjunctival flora and is therefore strongly recommended as a means of preventing postsurgical endophthalmitis following cataract surgery.
确定白内障手术患者的结膜细菌菌群以及术前用5%聚维酮碘溶液冲洗的效果。
在使用10 ml 5%聚维酮碘溶液冲洗前（T0）、冲洗后立即（T1）以及手术完成时（T2），从拟手术眼的下结膜穹窿处获取三个样本。将样本中的细菌接种于血琼脂、巧克力琼脂和硫乙醇酸盐肉汤中。
共有221例患者（n = 224只眼）纳入研究，平均年龄为67±13岁。来自（T0）样本的细菌在73.2%的硫乙醇酸盐琼脂、21.0%的血琼脂和19.2%的巧克力琼脂中成功生长。与T1样本相比，使用聚维酮碘冲洗使结膜定植减少了60.8%（从73.2%降至12.5%）（p<0.001），而T1和T2样本之间未发现显著差异。
用5%聚维酮碘溶液冲洗结膜可有效减少结膜菌群，因此强烈推荐将其作为预防白内障手术后眼内炎的一种手段。
