National Institute of Agrobiological Sciences, Owashi, Tsukuba, Ibaraki, Japan.
Arch Virol. 2010 Sep;155(9):1477-82. doi: 10.1007/s00705-010-0723-z. Epub 2010 Jun 13.
Dicistroviruses have motifs for picornavirus 2C, 3C, and 3D proteins in their nonstructural polyprotein C-terminal region. The proteins from the nonstructural, N-terminal region of the polyprotein remain to be characterized. We have identified 3C-mediated cleavage sites in the N-terminal region of the nonstructural polyprotein of the dicistrovirus Plautia stali intestine virus (PSIV). The 2B/2C cleavage site mapped to amino acids (aa) 408-409 (QD). 2B/2C cleavage sites were suggested to be conserved in dicistroviruses. The most N-terminal PSIV cleavage site was aa 286-287 (QS). Including previous results, the polyprotein contains nine proteins arranged as follows: 2A, 2B, 2C, 3A, 3B1, 3B2, 3B3, 3C, and 3D.
双顺反子病毒在其非结构多蛋白的 C 端区域具有小核糖核酸病毒 2C、3C 和 3D 蛋白的基序。多蛋白的非结构、N 端区域的蛋白仍有待表征。我们已经鉴定了双顺反子病毒 Plautia stali 肠病毒 (PSIV) 中非结构多蛋白 N 端区域的 3C 介导的切割位点。2B/2C 切割位点映射到氨基酸 (aa) 408-409 (QD)。据推测，2B/2C 切割位点在双顺反子病毒中是保守的。PSIV 的最 N 端切割位点为 aa 286-287 (QS)。包括以前的结果，该多蛋白包含以下排列的九个蛋白：2A、2B、2C、3A、3B1、3B2、3B3、3C 和 3D。
