Biotechnology Research Center, Kunming University of Science and Technology, Bailong Campus, Bailongsi, Bailong Road, Kunming, 650224, China.
Biotechnol Lett. 2010 Oct;32(10):1541-8. doi: 10.1007/s10529-010-0324-7. Epub 2010 Jun 15.
3-Hexulose-6-phosphate synthase (HPS) and 6-phosphate-3-hexuloisomerase (PHI) are two key enzymes in the formaldehyde (HCHO) assimilation pathway in methylotrophs. The HPS/PHI fusion protein, encoded by the chimeric gene of hps and phi from Mycobacterium gastri MB19, possesses both HPS and PHI activities in an Escherichia coli transformant. Overexpression of the fusion protein in chloroplasts of geranium (Pelargonium sp. Frensham) created a photosynthetic HCHO assimilation pathway according to (13)C-NMR analysis. The transgenic plants exhibited an enhanced ability in HCHO-uptake and [(14)C]HCHO-assimilation. Moreover, the transgenic plants showed greater HCHO-resistance and stronger capacity in purification of the HCHO-polluted air. Therefore, the use of the single chimeric gene may not only greatly simplify the transformation procedure but also improve the efficiency of phytoremediating HCHO in ornamental plants.
3-己酮糖-6-磷酸合酶（HPS）和 6-磷酸-3-己酮糖异构酶（PHI）是甲基营养菌中甲醛（HCHO）同化途径中的两种关键酶。来自胃分枝杆菌 MB19 的 hps 和 phi 嵌合基因编码的 HPS/PHI 融合蛋白在大肠杆菌转化体中具有 HPS 和 PHI 两种活性。通过（13）C-NMR 分析，在天竺葵（Pelargonium sp. Frensham）叶绿体中过量表达融合蛋白，创建了一种光合作用 HCHO 同化途径。转基因植物在 HCHO 摄取和[（14）C]HCHO 同化方面表现出增强的能力。此外，转基因植物表现出更高的 HCHO 抗性和更强的净化 HCHO 污染空气的能力。因此，使用单个嵌合基因不仅可以大大简化转化过程，而且可以提高观赏植物中 HCHO 植物修复的效率。
