DU Xue-Jun, Lei Yan, Yang Jing
Department of Cardiovascular Diseases, Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing 100091, China.
Zhong Xi Yi Jie He Xue Bao. 2010 Jun;8(6):548-53. doi: 10.3736/jcim20100607.
To investigate the effects of Radix Ginseng and Radix Notoginseng formula on expressions of vascular endothelial growth factor receptor-2 (VEGFR-2) and hypoxia-inducible factor-1alpha (HIF-1alpha) in ischemic myocardium of rats with acute myocardial infarction.
A total of 100 Wistar rats were randomly divided into normal control group, sham-operated group, untreated group, metoprolol (Betaloc) group, and high- and low-dose Radix Ginseng and Radix Notoginseng formula groups. Acute myocardial infarction was induced in the untreated group, Betaloc group, and high- and low-dose Radix Ginseng and Radix Notoginseng formula groups by left anterior descending coronary artery ligation. After 12-day treatment, microvessel density (MVD) in ischemic myocardium was detected by immunohistochemical method, while expressions of VEGFR-2 and HIF-1alpha proteins were detected by Western blotting, and expressions of VEGFR-2 and HIF-1alpha mRNAs were detected by real-time fluorescent quantitative polymerase chain reaction.
MVD in the untreated group was increased significantly, higher than those in the normal control group and the sham-operated group (P<0.05) and lower than those in the high- and low-dose Radix Ginseng and Radix Notoginseng formula groups and Betaloc group (P<0.01). VEGFR-2 and HIF-1alpha protein and mRNA expressions in the untreated group were higher than those in the normal control group and the sham-operated group (P<0.05). VEGFR-2 and HIF-1alpha protein and mRNA expressions in the high- and low-dose Radix Ginseng and Radix Notoginseng formula groups and Betaloc group were higher than those in the untreated group (P<0.05). There was a significant difference between the high- and low-dose Radix Ginseng and Radix Notoginseng formula groups (P<0.05).
Radix Ginseng and Radix Notoginseng extract can up-regulate the protein and mRNA expressions of VEGFR-2 and HIF-1alpha and increase MVD in ischemic myocardium to improve myocardial ischemia so as to promote the development of collateral circulation.
探讨人参三七配方对急性心肌梗死大鼠缺血心肌中血管内皮生长因子受体-2（VEGFR-2）和缺氧诱导因子-1α（HIF-1α）表达的影响。
将100只Wistar大鼠随机分为正常对照组、假手术组、未治疗组、美托洛尔（倍他乐克）组以及人参三七配方高、低剂量组。未治疗组、倍他乐克组以及人参三七配方高、低剂量组通过结扎左冠状动脉前降支诱导急性心肌梗死。治疗12天后，采用免疫组化法检测缺血心肌中的微血管密度（MVD），采用蛋白质印迹法检测VEGFR-2和HIF-1α蛋白的表达，采用实时荧光定量聚合酶链反应检测VEGFR-2和HIF-1α mRNA的表达。
未治疗组的MVD显著增加，高于正常对照组和假手术组（P<0.05），低于人参三七配方高、低剂量组和倍他乐克组（P<0.01）。未治疗组中VEGFR-2和HIF-1α蛋白及mRNA表达高于正常对照组和假手术组（P<0.05）。人参三七配方高、低剂量组和倍他乐克组中VEGFR-2和HIF-1α蛋白及mRNA表达高于未治疗组（P<0.05）。人参三七配方高、低剂量组之间存在显著差异（P<0.05）。
人参三七提取物可上调VEGFR-2和HIF-1α的蛋白及mRNA表达，增加缺血心肌中的MVD，改善心肌缺血，从而促进侧支循环的形成。
