Portela-Gomes Guida Maria, Grimelius Lars, Stridsberg Mats
Department of Gastroenterology, University of Lisbon, Lisbon, Portugal.
Regul Pept. 2010 Nov 30;165(1):30-5. doi: 10.1016/j.regpep.2010.06.002. Epub 2010 Jun 12.
Different epitopes of the granin family of proteins, chromogranin (Cg) A, CgB and secretogranin (Sg) II, have been demonstrated in normal human pancreas, gastrointestinal tract, adrenal medulla and in several neuroendocrine tumours (NETs). SgIII has been recently reported in endocrine pancreas. The aim of the present study was to examine the expression of SgIII in different NETs and compare it with the expression of CgA, CgB and SgII epitopes.
Tissue specimens from 47 NETs were analyzed. Antibodies to CgA 250-284, CgB 244-255, SgII 172-186 (C-terminal secretoneurin) and SgIII 348-361 were used for immunostaining.
SgIII was expressed in 41 of 47 NETs. The expression of SgIII agreed well with that of CgA, CgB and SgII, with exceptions of phaeochromocytomas, where more CgB and SgII immunoreactive cells were observed and parathyroid adenomas, which were only stained by CgA. In rectal NETs more cells expressed SgIII than CgA.
This is the first report on SgIII expression in various NETs. A majority of tumours studied displayed SgIII immunostaining, which indicates a functional relationship with the other granins.
在正常人类胰腺、胃肠道、肾上腺髓质以及几种神经内分泌肿瘤（NETs）中已证实存在嗜铬粒蛋白（Cg）A、CgB和分泌粒蛋白（Sg）II等嗜铬粒蛋白家族不同表位。最近有报道称内分泌胰腺中存在SgIII。本研究旨在检测SgIII在不同NETs中的表达，并将其与CgA、CgB和SgII表位的表达进行比较。
分析了47例NETs的组织标本。使用针对CgA 250 - 284、CgB 244 - 255、SgII 172 - 186（C端分泌素）和SgIII 348 - 361的抗体进行免疫染色。
47例NETs中有41例表达SgIII。SgIII的表达与CgA、CgB和SgII的表达情况基本一致，但嗜铬细胞瘤除外，其中观察到更多CgB和SgII免疫反应性细胞，甲状旁腺腺瘤仅被CgA染色。在直肠NETs中，表达SgIII的细胞比表达CgA的细胞更多。
这是关于SgIII在各种NETs中表达的首次报道。大多数所研究的肿瘤显示SgIII免疫染色，这表明其与其他嗜铬粒蛋白存在功能关系。
