Wang H, Ascoli M, Segaloff D L
Department of Physiology and Biophysics, University of Iowa, Iowa City 52242.
Endocrinology. 1991 Jul;129(1):133-8. doi: 10.1210/endo-129-1-133.
It has previously been shown that multiple messenger RNA (mRNA) species can be identified in gonadal tissues by probes specific for the LH/CG receptor. Here we show that the sizes and relative abundancies of gonadal LH/CG receptor transcripts are quite variable between such closely related species as rat and mouse. These patterns of LH/CG receptor mRNAs are yet different from that observed in human embryonic kidney 293 cells that have been transfected with a cDNA encoding for the rat luteal LH/CG receptor. In spite of the diversity in the number and sizes of LH/CG receptor mRNA transcripts, however, our data also show that the size of the cell surface receptor expressed in these three cells/tissues is identical. We further show that the most abundant LH/CG receptor mRNA present in MA-10 cells, a clonal strain of cultured Leydig tumor cells, is a 1.2 kilobase transcript which encodes for a truncated version of the LH/CG receptor corresponding to the extracellular hormone-binding domain. It does not appear, however, that this transcript is translated into a functional protein.
此前已有研究表明，通过针对促黄体生成素/绒毛膜促性腺激素（LH/CG）受体的特异性探针，可以在性腺组织中鉴定出多种信使核糖核酸（mRNA）种类。在此我们表明，在大鼠和小鼠等亲缘关系密切的物种之间，性腺LH/CG受体转录本的大小和相对丰度存在很大差异。这些LH/CG受体mRNA的模式与在转染了编码大鼠黄体LH/CG受体的cDNA的人胚肾293细胞中观察到的不同。然而，尽管LH/CG受体mRNA转录本的数量和大小存在差异，但我们的数据还表明，在这三种细胞/组织中表达的细胞表面受体的大小是相同的。我们进一步表明，在MA-10细胞（一种培养的睾丸间质细胞瘤细胞克隆株）中存在的最丰富的LH/CG受体mRNA是一种1.2千碱基的转录本，它编码LH/CG受体的截短版本，对应于细胞外激素结合结构域。然而，这种转录本似乎并没有被翻译成功能性蛋白质。
