USER克隆与USER融合：大小实验室的理想克隆技术。

USER cloning and USER fusion: the ideal cloning techniques for small and big laboratories.

作者信息

Nour-Eldin Hussam H, Geu-Flores Fernando, Halkier Barbara A

机构信息

Department of Plant Biology and Biotechnology, and VKR Centre Pro-Active Plants, Faculty of Life Sciences, University of Copenhagen, Frederiksberg, Denmark.

出版信息

Methods Mol Biol. 2010;643:185-200. doi: 10.1007/978-1-60761-723-5_13.

DOI:10.1007/978-1-60761-723-5_13

PMID:20552452

Abstract

The explosive development of the field of molecular biology has led to the need for simpler and more efficient cloning techniques. These requirements are elegantly met by the ligation-free cloning technique called USER cloning. USER cloning is suitable not only for everyday and high-throughput cloning but also for the one-step construction of complex DNA constructs, which can be achieved in a variant called USER fusion. In this chapter, we present a general protocol for converting any vector into a USER-compatible vector, together with protocols for both USER cloning and USER fusion.

摘要

分子生物学领域的迅猛发展使得对更简单、高效的克隆技术的需求日益凸显。名为USER克隆的无连接酶克隆技术出色地满足了这些要求。USER克隆不仅适用于日常和高通量克隆，还适用于复杂DNA构建体的一步法构建，这在一种称为USER融合的变体中能够实现。在本章中，我们将介绍把任何载体转化为USER兼容载体的通用方案，以及USER克隆和USER融合的方案。