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利用混合培养发酵从麦秆水解物中生产极端嗜热生物氢气:反应器构型的影响。

Extreme thermophilic biohydrogen production from wheat straw hydrolysate using mixed culture fermentation: effect of reactor configuration.

机构信息

Department of Environmental Engineering, Technical University of Denmark, DK-2800 Lyngby, Denmark.

出版信息

Bioresour Technol. 2010 Oct;101(20):7789-96. doi: 10.1016/j.biortech.2010.05.024.

DOI:10.1016/j.biortech.2010.05.024
PMID:20554199
Abstract

Hydrogen production from hemicellulose-rich wheat straw hydrolysate was investigated in continuously-stirred tank reactor (CSTR), up-flow anaerobic sludge bed (UASB) reactor, and anaerobic filter (AF) reactor. The CSTR was operated at an hydraulic retention time (HRT) of 3 days, and the UASB and AF reactors were operated at 1 day HRT, using mixed extreme thermophiles at 70 °C. The highest hydrogen production yield of 212.0±6.6 mL-H₂/g-sugars, corresponding to a hydrogen production rate of 821.4±25.5 mL-H₂/dL was achieved with the UASB reactor. Lowering the HRT to 2.5 days caused cell mass washout in the CSTR, while the UASB and AF reactors gave fluctuating and reducing hydrogen production at a 0.5-day HRT. The original rate and yield were recovered when the HRT was increased back to 1 day. These results demonstrate that reactor configuration is an important factor for enhancing and stabilizing H₂ production.

摘要

从富含半纤维素的小麦秸秆水解物中生产氢气,在连续搅拌釜式反应器(CSTR)、上流式厌氧污泥床(UASB)反应器和厌氧滤池(AF)反应器中进行了研究。CSTR 的水力停留时间(HRT)为 3 天,UASB 和 AF 反应器的 HRT 为 1 天,在 70°C 下使用混合极端嗜热菌。在 UASB 反应器中,最高产氢率为 212.0±6.6 mL-H₂/g-糖,对应的产氢速率为 821.4±25.5 mL-H₂/dL。将 HRT 降低至 2.5 天会导致 CSTR 中细胞质量洗脱,而 UASB 和 AF 反应器在 HRT 为 0.5 天时会出现产氢波动和减少。当 HRT 增加回 1 天时,原始的速率和产率得到恢复。这些结果表明,反应器配置是增强和稳定氢气生产的重要因素。

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