边缘毛刺蟹血淋巴中法尼醇甲酯结合蛋白的特性分析

Characterization of a methyl farnesoate binding protein in hemolymph from Libinia emarginata.

作者信息

Li H, Borst D W

机构信息

Department of Biological Sciences, Illinois State University, Normal 61761.

出版信息

Gen Comp Endocrinol. 1991 Mar;81(3):335-42. doi: 10.1016/0016-6480(91)90159-4.

DOI:10.1016/0016-6480(91)90159-4

PMID:2055434

Abstract

Hemolymph from Libinia emarginata was tested for its ability to bind methyl farnesoate (MF), a JH-like compound found in many crustaceans. Hemolymph bound MF with moderate affinity (KD = 4.5 x 10(-6) M). Competitive binding studies showed that this binding was specific for MF, with farnesoic acid and JH homologues having less than 30 and 7%, respectively, of the relative binding activity of MF. JH acid and ecdysterone had no binding activity. MF binding activity was lost after pretreatment of hemolymph with heat or protease, suggesting that the binding component was a protein. Gel filtration analysis showed that the binding activity had a molecular weight of about 650,000.

摘要

对缘毛哲蟹的血淋巴进行了检测，以评估其结合法尼酸甲酯（MF）的能力，MF是一种在许多甲壳类动物中发现的类保幼激素化合物。血淋巴以中等亲和力（KD = 4.5×10⁻⁶ M）结合MF。竞争性结合研究表明，这种结合对MF具有特异性，法尼酸和保幼激素同系物的相对结合活性分别不到MF的30%和7%。保幼激素酸和蜕皮甾酮没有结合活性。用热或蛋白酶预处理血淋巴后，MF结合活性丧失，这表明结合成分是一种蛋白质。凝胶过滤分析表明，结合活性的分子量约为650,000。