甜菜卫星DNA家族的克隆与特征分析
Cloning and characterization of a Beta vulgaris satellite DNA family.
作者信息
Schmidt T, Metzlaff M
机构信息
Institute of Genetics, Martin-Luther-University, Halle/Saale, F.R.G.
出版信息
Gene. 1991 May 30;101(2):247-50. doi: 10.1016/0378-1119(91)90418-b.
Three members of the BamHI-sequence family of sugar beet (Beta vulgaris) have been cloned in Escherichia coli and compared by sequence analysis. The sequence family shows the typical features of eukaryotic satellite DNA, e.g., organization in tandem arrays and sequence divergence. A typical ladder pattern of a monomer (327 bp) and multiple oligodeoxyribonucleotides have been observed. The BamHI monomer is A + T-rich (69%) and does not show any similarity to other known plant satellite DNAs.
甜菜（Beta vulgaris）BamHI序列家族的三个成员已在大肠杆菌中克隆，并通过序列分析进行了比较。该序列家族显示出真核卫星DNA的典型特征，例如串联排列的组织形式和序列差异。观察到了单体（327 bp）和多个寡脱氧核糖核苷酸的典型梯状模式。BamHI单体富含A + T（69%），与其他已知的植物卫星DNA没有任何相似性。
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