Fraunhofer Institute for Cell Therapy and Immunology (IZI), Perlickstraße 01, 04103 Leipzig, Germany.
Cell Mol Life Sci. 2010 Dec;67(23):4095-106. doi: 10.1007/s00018-010-0427-2. Epub 2010 Jun 18.
Non-adherent bone marrow-derived cells (NA-BMCs) are a mixed cell population that can give rise to multiple mesenchymal phenotypes and that facilitates hematopoietic recovery. We characterized NA-BMCs by flow cytometry, fibroblast colony-forming units (CFU-f), real-time PCR, and in in vivo experiments. In comparison to adherent cells, NA-BMCs expressed high levels of CD11b(+) and CD90(+) within the CD45(+) cell fraction. CFU-f were significantly declining over the cultivation period, but NA-BMCs were still able to form CFU-f after 5 days. Gene expression analysis of allogeneic NA-BMCs compared to bone marrow (BM) indicates that NA-BMCs contain stromal, mesenchymal, endothelial cells and monocytes, but less osteoid, lymphoid, and erythroid cells, and hematopoietic stem cells. Histopathological data and analysis of weight showed an excellent recovery and organ repair of lethally irradiated mice after NA-BMC transplantation with a normal composition of the BM.
非黏附骨髓来源细胞(NA-BMCs)是一种混合细胞群体,能够产生多种间充质表型,并促进造血恢复。我们通过流式细胞术、成纤维细胞集落形成单位(CFU-f)、实时 PCR 以及体内实验对 NA-BMCs 进行了表征。与贴壁细胞相比,NA-BMCs 在 CD45+细胞群中高表达 CD11b(+)和 CD90(+)。CFU-f 在培养期间显著下降,但 NA-BMCs 仍能在 5 天后形成 CFU-f。与骨髓(BM)相比,同种异体 NA-BMCs 的基因表达分析表明,NA-BMCs 包含基质细胞、间充质细胞、内皮细胞和单核细胞,但骨样细胞、淋巴样细胞和红样细胞以及造血干细胞较少。组织病理学数据和体重分析表明,在致死性照射小鼠中进行 NA-BMC 移植后,BM 组成正常,其恢复和器官修复良好。