Kuszak J R, Sivak J G, Weerheim J A
Department of Pathology, Rush-Presbyterian-St. Luke's Medical Center, Chicago, IL 60612.
Invest Ophthalmol Vis Sci. 1991 Jun;32(7):2119-29.
We analyzed the structural and functional relationship between lens sutures and lens optical quality (focal length variability) by correlative scanning electron microscopy (SEM) and laser scan analysis. Twenty-two rabbit lenses (8 pigmented and 14 albino) were used in this study. Lenses were initially scanned by a low-power helium-neon laser beam that was passed either at an acute angle to a lens suture or along a lens suture. The results of laser scan analysis with the incident beam passed at an acute angle to a lens suture showed that generally, rabbit lenses were well corrected for spherical aberration. Subsequent SEM analysis showed that areas of lenses scanned that produced the least amount of focal variability were characterized by uniform fiber cells arranged in parallel, radial cell columns. In contrast, the results of laser scan analysis with the incident beam passed along a lens suture showed that there was significant focal length variability, i.e., spherical aberration at the lens sutures. Subsequent SEM analysis showed that the areas of lenses scanned that produced the greatest amounts of focal variability (lens sutures) were characterized by nonuniform fiber cell ends arranged as erratic suture branches in single growth shells and collectively as erratic suture planes formed between growth shells extending from the embryonic nucleus to the lens periphery. Furthermore, the amount of focal variability was directly proportional to the degree of structural disorder at the lens sutures. This is the first study to unequivocally show that the relationship between lens optical quality and specific parameters of lens morphology (lens sutures) can be quantified. These findings may help to elucidate the pathologic changes that lead to presbyopia and cortical cataractogenesis because these lenses are characterized by asymmetrical suture patterns and planes.
我们通过相关扫描电子显微镜（SEM）和激光扫描分析，分析了晶状体缝线与晶状体光学质量（焦距变异性）之间的结构和功能关系。本研究使用了22只兔眼晶状体（8只有色的和14只白化的）。首先用低功率氦氖激光束对晶状体进行扫描，激光束以锐角穿过晶状体缝线或沿着晶状体缝线传播。当入射光束以锐角穿过晶状体缝线进行激光扫描分析的结果表明，一般来说，兔眼晶状体的球差得到了很好的校正。随后的SEM分析表明，扫描的晶状体区域中产生最小焦距变异性的区域，其特征是纤维细胞排列均匀，呈平行的放射状细胞柱。相比之下，当入射光束沿着晶状体缝线传播进行激光扫描分析的结果表明，存在显著的焦距变异性，即晶状体缝线处存在球差。随后的SEM分析表明，扫描的晶状体区域中产生最大焦距变异性的区域（晶状体缝线），其特征是纤维细胞末端不均匀，在单个生长壳中排列成不规则的缝线分支，在从胚胎核延伸到晶状体周边的生长壳之间共同形成不规则的缝线平面。此外，焦距变异性的大小与晶状体缝线处的结构紊乱程度成正比。这是第一项明确表明晶状体光学质量与晶状体形态学特定参数（晶状体缝线）之间的关系可以量化的研究。这些发现可能有助于阐明导致老花眼和皮质性白内障形成的病理变化，因为这些晶状体的特征是缝线模式和平面不对称。
