Department of Histopathology, King Edward Memorial Hospital, University of Western Australia, Perth, Australia.
Histopathology. 2010 Jul;57(1):46-54. doi: 10.1111/j.1365-2559.2010.03588.x. Epub 2010 Jun 14.
To investigate micro-anatomical variations in proliferative activity within uterine endometrioid adenocarcinoma with particular emphasis on tumour areas comprising microcystic, elongated and fragmented ('MELF') glands.
Ki67 immunoreactivity was assessed in 29 low-grade endometrial adenocarcinomas specifically comparing conventional tumour glands and areas exhibiting MELF-type alteration. Furthermore, since Ki67 expression differed between the peripheral and central aspects of larger neoplastic glands, these micro-anatomical compartments were assessed separately using a semiquantitative scoring system. Most MELF-type tumour elements were negative for Ki67 or showed only rare (< 5% cells) positivity. In contrast, peripheral conventional tumour glands showed prominent Ki67 labelling, but this was significantly reduced in central glandular areas. An inverse correlation between Ki67 and cytokeratin (CK) 7 expression was noted in many tumours.
Endometrial adenocarcinomas show micro-anatomical variations in Ki67 expression and this is often inversely correlated with CK7 immunoreactivity. MELF-type tumour elements show minimal proliferative activity, a finding that initially appears unexpected for areas of purported active invasion. However, an inverse correlation between cell division and local invasion has been demonstrated in other malignancies, notably during epithelial-mesenchymal transition, and this may reflect a reversible alteration in cellular activity during neoplastic progression.
研究子宫内膜样腺癌中增殖活性的微观解剖学变化，特别强调包含微囊状、拉长和碎片化（“MELF”）腺体的肿瘤区域。
在 29 例低级别子宫内膜腺癌中评估了 Ki67 免疫反应性，特别比较了常规肿瘤腺体和表现出 MELF 型改变的区域。此外，由于 Ki67 表达在较大肿瘤腺体的外周和中央方面存在差异，因此使用半定量评分系统分别评估这些微观解剖学隔室。大多数 MELF 型肿瘤成分 Ki67 阴性或仅显示罕见（<5%细胞）阳性。相比之下，外周常规肿瘤腺体显示出明显的 Ki67 标记，但在中央腺区明显减少。在许多肿瘤中观察到 Ki67 与细胞角蛋白（CK）7 表达之间的负相关。
子宫内膜腺癌在 Ki67 表达方面存在微观解剖学变化，这通常与 CK7 免疫反应性呈负相关。MELF 型肿瘤成分增殖活性低，对于推测为活跃侵袭的区域，这一发现最初似乎出乎意料。然而，在其他恶性肿瘤中已经证明了细胞分裂与局部侵袭之间的反向相关性，特别是在上皮-间充质转化期间，这可能反映了肿瘤进展过程中细胞活性的可逆改变。
