School of Human Science and Environment, University of Hyogo, Himeji 670-0092, Japan.
Arch Biochem Biophys. 2010 Sep 15;501(2):182-7. doi: 10.1016/j.abb.2010.06.010. Epub 2010 Jun 15.
Research into lipid peroxidation-induced protein modification has been ongoing for many years. Recent studies on lipo-oxidation shows the occurrence of another type of protein modification, amide-type adduct formation by lipid hydroperoxide, as well as classical aldehyde-derived protein modifications. The amide-type modifications can be either classified as alkylamide and carboxyalkylamide according to the formed structures. As an alkylamide-type adduct, Nepsilon-(hexanoyl)lysine can be formed by the reaction of peroxidized n-6 fatty acid with lysine. Nepsilon-(propanoyl)lysine is considered to be generated from oxidation of n-3 fatty acid with lysine. The generation pattern of both might be useful for classification of which fatty acids are more involved in oxidation in vivo. Since the alkylamide type-adducts are relatively stable and detectable from biological specimens like urine, these adducts, especially Nepsilon-(hexanoyl)lysine, are used as reliable markers for not only oxidative stress evaluation but also development of functional food.
对脂质过氧化诱导的蛋白质修饰的研究已经进行了多年。最近的脂质氧化研究表明，另一种类型的蛋白质修饰，即脂质氢过氧化物的酰胺型加合物形成，以及经典的醛衍生的蛋白质修饰的发生。根据形成的结构，酰胺型修饰可以分为烷基酰胺和羧基烷基酰胺。作为一种烷基酰胺型加合物，Nepsilon-（己酰）赖氨酸可以通过过氧化 n-6 脂肪酸与赖氨酸的反应形成。Nepsilon-（丙酰）赖氨酸被认为是由 n-3 脂肪酸与赖氨酸氧化生成的。这两种物质的生成模式可能有助于对体内哪种脂肪酸更易氧化进行分类。由于烷基酰胺型加合物相对稳定，并且可以从尿液等生物标本中检测到，因此这些加合物，特别是 Nepsilon-（己酰）赖氨酸，不仅可用作氧化应激评估的可靠标志物，还可用作功能性食品的开发。
