使用局部小干扰 RNA 对多巴胺转运体敲低小鼠进行行为表型分析。
Behavioural phenotyping of dopamine transporter knockdown mice using local small interference RNA.
机构信息
Department of Psychology, National Taiwan University, Taipei, Taiwan.
出版信息
Behav Brain Res. 2010 Dec 25;214(2):475-9. doi: 10.1016/j.bbr.2010.06.008. Epub 2010 Jun 15.
The objective of this study was to evaluate basic behavioural phenotypes and methamphetamine-induced hyperactivity in dopamine transporter (DAT) knockdown and control mice after local injections of small interference RNA into ventral tegmental area/substantia nigra once every other day for 14 days. Local knockdown of DAT expression in the mouse brain did not affect basic behavioural functions, but these mice displayed elevated depression-like immobility and a approximately 35% reduction of methamphetamine-induced hyperactivity.
本研究的目的是评估多巴胺转运体（DAT）敲低和对照小鼠的基本行为表型和 methamphetamine 诱导的过度活动，方法是在 14 天内每隔一天向腹侧被盖区/黑质局部注射小干扰 RNA。在小鼠大脑中局部敲低 DAT 表达不会影响基本行为功能，但这些小鼠表现出更高的抑郁样不动性和 methamphetamine 诱导的过度活动减少约 35%。
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