Senra Marcus V X, Vizzoni Vinicius F, Trindade-Silva Amaro E, Giannini Ana L M, Soares Carlos A G
Laboratório de Genética Molecular de Eucariontes e Simbiontes, Departamento de Genética, Instituto de Biologia, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.
J Mol Microbiol Biotechnol. 2010;18(4):215-9. doi: 10.1159/000316422. Epub 2010 Jun 18.
Teredinibacter turnerae belongs to a group of biotechnologically relevant bacteria. Gene transfer into T. turnerae was achieved by using pPROBE'-gfp[ASV] derived plasmids through conjugative mating with Escherichia coli DH5alpha pRK2073. Transferred plasmids were stably maintained and T. turnerae could also act as a donor to transfer these mobilizable plasmids. Constructs for both constitutive and IPTG-inducible gene expression were obtained, representing new tools for gene overexpression in T. turnerae.
特纳氏栖木杆菌属于一组具有生物技术相关性的细菌。通过使用源自pPROBE'-gfp[ASV]的质粒与大肠杆菌DH5α pRK2073进行接合交配，实现了基因向特纳氏栖木杆菌的转移。转移的质粒能够稳定维持，并且特纳氏栖木杆菌也可以作为供体来转移这些可移动质粒。获得了组成型和IPTG诱导型基因表达的构建体，它们代表了在特纳氏栖木杆菌中进行基因过表达的新工具。
