Pettersson K J, Olsson A
Department of Bioanalysis, Astra Research Centre AB, Södertälje, Sweden.
J Chromatogr. 1991 Feb 15;563(2):414-8. doi: 10.1016/0378-4347(91)80050-m.
A reversed-phase high-performance liquid chromatographic method has been developed for the determination of the R- and S-enantiomers of ibuprofen. The enantiomers and the internal standard 4-pentylphenylacetic acid are extracted from plasma, separated and quantified on a Chiral-AGP column using ultraviolet detection. The simplicity, sensitivity and precision of the method makes it convenient for use in pharmacokinetic studies.
已开发出一种反相高效液相色谱法用于测定布洛芬的R-和S-对映体。对映体和内标4-戊基苯乙酸从血浆中萃取出来，在Chiral-AGP柱上采用紫外检测进行分离和定量。该方法的简便性、灵敏度和精密度使其便于用于药代动力学研究。
