Bavarian State Collection of Zoology, Münchhausenstrasse 21, München, Germany.
Micron. 2010 Oct;41(7):886.e1-886.e17. doi: 10.1016/j.micron.2010.03.010. Epub 2010 Apr 2.
We present three examples of interactive, 3D volume rendering models embedded in a PDF publication. The examples are drawn from three different morphological methods - confocal microscopy, serial sectioning and microcomputed tomography - performed on members of the phylum Mollusca. A description of the entire technical procedure from specimen preparation to embedding of the visual model including 3D labels in the document is provided. For comparison, volume rendering with standard visualization software, and surface rendering incorporated in the 3D PDF figures, are provided. The principal advantages and disadvantages of the techniques and models are discussed. Volume rendering for serial sections is relatively work-intensive, while confocal data have limitations in terms of 3D presentation. Volume renderings are normally downsampled in resolution to achieve a reasonable PDF file size, however intentional information is largely retained. We conclude that volume rendering of 3D data sets is a valuable technique and should become standard in PDF versions of biomedical publications.
我们展示了三个嵌入 PDF 出版物中的交互式 3D 体积渲染模型的示例。这些示例来自门软体动物的三个不同的形态学方法——共聚焦显微镜、连续切片和微计算机断层扫描。提供了从标本制备到嵌入文档中的 3D 标签的整个技术过程的描述。为了进行比较，还提供了标准可视化软件的体积渲染和 3D PDF 图形中包含的表面渲染。讨论了这些技术和模型的主要优缺点。对于连续切片的体积渲染相对来说比较费力，而共聚焦数据在 3D 呈现方面存在局限性。为了实现合理的 PDF 文件大小，通常会对体积渲染进行分辨率下采样，但是会保留大部分有意的信息。我们得出结论，3D 数据集的体积渲染是一种有价值的技术，应该成为生物医学出版物的 PDF 版本中的标准。
