Huck Institute for the Life Sciences, Penn State University, University Park, PA 16803, USA.
Bioinformatics. 2010 Jul 15;26(14):1783-5. doi: 10.1093/bioinformatics/btq281. Epub 2010 Jun 18.
Here, we describe a tool suite that functions on all of the commonly known FASTQ format variants and provides a pipeline for manipulating next generation sequencing data taken from a sequencing machine all the way through the quality filtering steps.
This open-source toolset was implemented in Python and has been integrated into the online data analysis platform Galaxy (public web access: http://usegalaxy.org; download: http://getgalaxy.org). Two short movies that highlight the functionality of tools described in this manuscript as well as results from testing components of this tool suite against a set of previously published files are available at http://usegalaxy.org/u/dan/p/fastq
在这里,我们描述了一个工具套件,它可以处理所有常见的 FASTQ 格式变体,并提供了一个从测序仪获取下一代测序数据的管道,一直到质量过滤步骤。
这个开源工具集是用 Python 实现的,并已集成到在线数据分析平台 Galaxy 中(公共网络访问:http://usegalaxy.org;下载:http://getgalaxy.org)。有两个短视频,突出了本文描述的工具的功能,以及使用该工具套件的组件对一组以前发布的文件进行测试的结果,可在 http://usegalaxy.org/u/dan/p/fastq 上查看。