Department of Cell Biology, and Department of Neurobiology, Duke University Medical Center, Durham, NC, USA.
Autophagy. 2010 Aug;6(6):798-9. doi: 10.4161/auto.6.6.12511. Epub 2010 Aug 28.
PIK3C3/Vps34 plays important roles in the endocytic and autophagic pathways, both of which are essential for maintaining neuronal integrity. However, it is unclear how inactivating PIK3C3 may affect neuronal endosomal versus autophagic processes in vivo. We generated a conditional null allele of the Pik3c3 gene in mouse, and specifically deleted it in postmitotic sensory neurons. Subsequent analyses reveal several interesting and surprising findings.
PIK3C3/Vps34 在胞吞作用和自噬途径中发挥重要作用,这两个途径对于维持神经元完整性都是必不可少的。然而,尚不清楚失活 PIK3C3 如何影响体内神经元内体与自噬过程。我们在小鼠中生成了 Pik3c3 基因的条件性缺失等位基因,并在有丝分裂后感觉神经元中特异性地删除了它。随后的分析揭示了一些有趣和令人惊讶的发现。