用于激光诱导荧光检测的集成微流控生物光子学芯片。

Integrated microfluidic biophotonic chip for laser induced fluorescence detection.

机构信息

Optical Bio Microsystems Laboratory, Department of Mechanical Engineering, Concordia University, Montreal, QC, Canada.

出版信息

Biomed Microdevices. 2010 Oct;12(5):923-33. doi: 10.1007/s10544-010-9447-9.

PMID:20563752

Abstract

Integrated Lab-on-a-Chip or Micro-Total Analysis Systems offer several advantages for the detection of active chemical and biological species. In this work, an integrated microfluidic biophotonic chip is proposed for carrying out laser induced fluorescence detection. A Spectrometer-on-Chip device, specifically designed for multiple fluorescence detections at different emission wavelengths is integrated with the opto-microfluidic chip fabricated on Silicon-Polymer hybrid platform. The input fiber from the laser source, and output fiber coupled with a Spectrometer-on-Chip were integrated with the microfluidic channel so as to make a robust setup. Fluorescence detection was carried out using Alexafluor 647 tagged antibody particles. The experimental results show that the proposed biophotonic microfluidic device is highly suitable for high throughput detection of chemical and biological specimens.

摘要

集成式微流控芯片或微全分析系统为活性化学和生物种类的检测提供了多项优势。在这项工作中，提出了一种用于进行激光诱导荧光检测的集成微流控生物光子芯片。与在硅-聚合物混合平台上制造的光电微流控芯片集成的是专门用于在不同发射波长下进行多次荧光检测的片上光谱仪设备。来自激光源的输入光纤和与片上光谱仪耦合的输出光纤与微流道集成在一起，以构成一个坚固的装置。使用 Alexafluor 647 标记的抗体颗粒进行荧光检测。实验结果表明，所提出的生物光子微流控器件非常适合用于高通量检测化学和生物样本。