Irawan Rudi, Tjin Swee Chuan, Fang Xiaoqin, Fu Chit Yaw
BioMedical Engineering Research Centre, Nanyang Technological University, Singapore 637553, Singapore.
Biomed Microdevices. 2007 Jun;9(3):413-9. doi: 10.1007/s10544-007-9052-8.
A combination of fluorescence detection and microfluidic technology provides promising applications in life sciences. A prototype of an integrated fluorescence detection system and optical fiber light guide on a laminate-based multichannel microfluidic chip has been developed and tested. A blue LED, plastic optical fiber, photodiode, Mylar and PMMA, and fluorescein and BSA-FITC were used as an excitation source, light coupler and guide, detector, microfluidic substrate and sample, respectively. The results show that the system is capable of detecting weak fluorescence emission from a fluorescein solution at concentration down to 0.01 ng/ml, and gives linear response. The results were also reproducible, and no cross-talk between adjacent channels was observed. The test using BSA as a model analyte demonstrates its feasibility for on-chip immunosensor applications. The performance and applications can be developed further. This prototype can be used as a platform to develop a simple and compact bio-fluorescence detection system integrated with an inexpensive and disposable multichannel microfluidic chip for biomedical devices.
荧光检测与微流控技术的结合在生命科学领域有着广阔的应用前景。一种基于层压多通道微流控芯片的集成荧光检测系统和光纤光导的原型已经被开发并测试。分别使用蓝色发光二极管、塑料光纤、光电二极管、聚酯薄膜和聚甲基丙烯酸甲酯，以及荧光素和牛血清白蛋白-异硫氰酸荧光素作为激发源、光耦合器和光导、探测器、微流控基板和样品。结果表明，该系统能够检测浓度低至0.01 ng/ml的荧光素溶液的微弱荧光发射，并给出线性响应。结果具有可重复性，且未观察到相邻通道之间的串扰。以牛血清白蛋白作为模型分析物的测试证明了其在芯片上免疫传感器应用中的可行性。其性能和应用可以进一步开发。该原型可作为一个平台，用于开发一种简单紧凑的生物荧光检测系统，该系统集成了用于生物医学设备的廉价且一次性的多通道微流控芯片。
