Kaczmarek Agnieszka, Budzyńska Anna, Gospodarek Eugenia
Katedra i Zakład Mikrobiologii, Collegium Medicum im. Ludwika Rydygiera w Bydgoszczy, Uniwersytetu Mikołaja Kopernika w Toruniu.
Med Dosw Mikrobiol. 2010;62(1):59-65.
The aim of the study was to determine the frequency of occurrence of K1 surface antigen in Escherichia coli strains isolated from the pregnant women and newborns. A total of 425 of E. coli strains isolated from the faecal samples, 67 strains isolated from the vagina of pregnant women and 40 strains isolated from the newborns' nasal cavity were included into the study. All strains were collected between June and September of 2008. Identification of isolates was followed by the assessment of presence of K1 surface antigen in E. coli strains. The presence of K1 antigen was found in 17,6% of E. coli strains isolated from the faecal samples, 20,9% of E. coli strains isolated from the vagina of pregnant women and in 17,5% of E. coli strains isolated from the newborns' nasal cavity. Routine screening of E. coli K1 colonization gives an opportunity to identify women with the risk of E. coli K1 transmission to neonates during delivery and thereby with major probability of perinatal infections. Latex agglutination test Pastorex Meningitis (Bio-Rad) provides fast identification of E. coli K1 strains.
本研究的目的是确定从孕妇和新生儿中分离出的大肠杆菌菌株中K1表面抗原的出现频率。该研究纳入了从粪便样本中分离出的425株大肠杆菌菌株、从孕妇阴道中分离出的67株大肠杆菌菌株以及从新生儿鼻腔中分离出的40株大肠杆菌菌株。所有菌株均于2008年6月至9月间收集。对分离菌株进行鉴定后,评估大肠杆菌菌株中K1表面抗原的存在情况。在从粪便样本中分离出的大肠杆菌菌株中,17.6%检测到K1抗原;从孕妇阴道中分离出的大肠杆菌菌株中,20.9%检测到K1抗原;从新生儿鼻腔中分离出的大肠杆菌菌株中,17.5%检测到K1抗原。对大肠杆菌K1定植进行常规筛查,有助于识别在分娩期间有大肠杆菌K1传播给新生儿风险的女性,从而极大可能地预防围产期感染。Pastorex Meningitis乳胶凝集试验(伯乐公司)可快速鉴定大肠杆菌K1菌株。