Van Alphen L, Bol P, Kok M L, Geelen-Van Den Broek L
Department of Medical Microbiology, University of Amsterdam, The Netherlands.
J Med Microbiol. 1991 Jun;34(6):313-6. doi: 10.1099/00222615-34-6-313.
Twenty-four Haemophilus influenzae type b strains from 836 children and young adults in an open population were subtyped by outer-membrane-protein (OMP) analysis on sodium dodecyl sulphate-polyacrylamide gels, lipopolysaccharide serotyping and biotyping. The results were compared with those obtained with H. influenzae type b strains from 97 patients with meningitis in the same city (Amsterdam). OMP subtype 1 was significantly more common among the CSF isolates than in carrier strains (82% vs 50%; p less than 0.002). The other OMP subtypes found among carriers were rarely isolated from patients. The lipopolysaccharide serotype and biotype distribution did not differ between the two groups. The combination of OMP subtype 1, lipopolysaccharide 1, biotype I was much more common in isolates from patients than in those from carriers (71% vs 42%; p less than 0.01). The data suggest that various H. influenzae type b subtypes are less virulent than those commonly isolated from invasive infections.
对来自开放人群中836名儿童和年轻人的24株b型流感嗜血杆菌菌株，通过十二烷基硫酸钠-聚丙烯酰胺凝胶上的外膜蛋白（OMP）分析、脂多糖血清分型和生物分型进行亚型鉴定。将结果与来自同一城市（阿姆斯特丹）97例脑膜炎患者的b型流感嗜血杆菌菌株的结果进行比较。OMP亚型1在脑脊液分离株中比在携带菌株中显著更常见（82%对50%；p<0.002）。在携带者中发现的其他OMP亚型很少从患者中分离出来。两组之间脂多糖血清型和生物型分布没有差异。OMP亚型1、脂多糖1、生物型I的组合在患者分离株中比在携带者分离株中更常见（71%对42%；p<0.01）。数据表明，各种b型流感嗜血杆菌亚型的毒力低于通常从侵袭性感染中分离出的菌株。
