Laboratory of Influenza Research, College of Veterinary Medicine, Chungnam National University, Yuseong-Gu, Daejeon, Korea.
Viral Immunol. 2010 Jun;23(3):321-7. doi: 10.1089/vim.2009.0093.
Outbreaks of highly pathogenic H5N1 influenza viruses have been reported in many countries worldwide. The possibility of pandemics caused by H5N1 influenza viruses is high since human infections by H5N1 viruses continually occur. In this study we determined the immune response and efficacy of inactivated H5N1 vaccine developed by reverse genetics in ferrets. Ferrets intramuscularly inoculated with two doses of H5N1 vaccine survived the lethal challenge with homologous or heterologous H5N1 influenza viruses, while 75% and 25% of ferrets immunized with one dose of H5N1 vaccine survived the lethal challenge with homologous and heterologous H5N1 influenza viruses, respectively. When we determined antibody subtypes specific for H5N1 influenza viruses in tissues and sera of vaccinated ferrets, IgG antibodies were detected mainly in the trachea, nostril, lung, heart, liver, kidney, intestine, spleen, and serum. Our results suggest that IgG antibodies may play a major role in protecting ferrets immunized with the inactivated H5N1 vaccine from lethal challenge with H5N1 influenza viruses.
已在世界许多国家报告了高致病性 H5N1 流感病毒的暴发。由于人类不断感染 H5N1 病毒，因此由 H5N1 流感病毒引起大流行的可能性很高。在这项研究中，我们确定了通过反向遗传学开发的灭活 H5N1 疫苗在雪貂中的免疫反应和功效。用两剂 H5N1 疫苗肌肉接种的雪貂能够耐受同源或异源 H5N1 流感病毒的致死性挑战，而用一剂 H5N1 疫苗免疫的雪貂中，分别有 75%和 25%能够耐受同源和异源 H5N1 流感病毒的致死性挑战。当我们确定接种疫苗的雪貂的组织和血清中针对 H5N1 流感病毒的抗体亚型时，在气管、鼻腔、肺、心脏、肝脏、肾脏、肠、脾脏和血清中检测到 IgG 抗体。我们的结果表明，IgG 抗体可能在保护用灭活 H5N1 疫苗免疫的雪貂免受 H5N1 流感病毒的致死性挑战方面发挥主要作用。
