Cooper M L, Laxer J A, Hansbrough J F
Department of Surgery, University of California, San Diego Medical Center 92103.
J Trauma. 1991 Jun;31(6):775-82; discussion 782-4. doi: 10.1097/00005373-199106000-00007.
This study evaluated the cytotoxicity of commonly used topical agents to human dermal fibroblasts and epidermal keratinocytes, which play a prominent role in wound healing. The effects of these topical agents were assessed using two separate assays for the fibroblasts--tritiated thymidine incorporation and the uptake of a vital dye (neutral red). Keratinocytes were evaluated with the neutral red assay. Serial dilutions of each of 10 commonly used topical agents produced decreases in both the uptake of neutral red and the incorporation of thymidine at clinically relevant doses. Only Neosporin G.U. irrigant showed no significant difference compared with controls in the assays for both the fibroblasts and the keratinocytes. Careful attention must be paid to which agent is used in the clinical setting, since many of these can have profound effects on cells that influence wound healing.
本研究评估了常用外用剂对人真皮成纤维细胞和表皮角质形成细胞的细胞毒性，这两种细胞在伤口愈合中起着重要作用。使用两种针对成纤维细胞的独立检测方法——氚标记胸腺嘧啶核苷掺入法和一种活性染料（中性红）摄取法，评估这些外用剂的效果。角质形成细胞则用中性红检测法进行评估。10种常用外用剂的系列稀释液在临床相关剂量下均使中性红摄取量和胸腺嘧啶核苷掺入量降低。在成纤维细胞和角质形成细胞检测中，只有新孢霉素G.U.冲洗液与对照组相比无显著差异。在临床环境中必须谨慎选择使用哪种外用剂，因为其中许多外用剂会对影响伤口愈合的细胞产生深远影响。
