McCauley R L, Linares H A, Pelligrini V, Herndon D N, Robson M C, Heggers J P
Skin Bank, University of Texas Medical Branch, Galveston 77550.
J Surg Res. 1989 Mar;46(3):267-74. doi: 10.1016/0022-4804(89)90069-3.
Topical antimicrobial agents are essential to optimal burn care. However, exposure of WI-38 human diploid fibroblasts (ATCC CCL 75) and fresh donor human dermal fibroblasts to silver sulfadiazine and mafenide acetate results in a significant reduction in cell proliferation, as determined by hemocytometer cell counts and total matrix protein assays, within 48 hr of exposure. Changes in cellular morphology and progressive deterioration of cytoplasmic organelles and the nucleus are seen with phase-contrast microscopy and transmission electron microscopy. These findings may explain the clinical observation of delayed wound healing after the use of topical antimicrobial agents.
局部抗菌剂对于优化烧伤护理至关重要。然而，通过血细胞计数器细胞计数和总基质蛋白测定发现，WI-38人二倍体成纤维细胞（ATCC CCL 75）和新鲜供体人真皮成纤维细胞在暴露于磺胺嘧啶银和醋酸磺胺米隆48小时内，细胞增殖显著减少。相差显微镜和透射电子显微镜观察到细胞形态的变化以及细胞质细胞器和细胞核的逐渐退化。这些发现可能解释了使用局部抗菌剂后伤口愈合延迟的临床观察结果。
